FRET and FLIM TechniquesThis volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields. |
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Índice
| 1 | |
| 59 | |
| 95 | |
| 133 | |
Visible fluorescent proteins for FRET | 171 |
Small moleculebased FRET probes | 225 |
FilterFRET Quantitative imaging of sensitized emission | 289 |
Spectral imaging and its use in the measurement of Foumlrster resonance energy transfer in living cells | 351 |
Total internal reflection fluorescence lifetime imaging microscopy | 395 |
FRET and FLIM applications in plants | 413 |
Biomedical FRETFLIM applications | 447 |
Reflections on FRET imaging Formalism probes and implementation | 475 |
Glossary | 519 |
Index | 521 |
Color Plate Section | 535 |
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Palavras e frases frequentes
acceptor photobleaching acquisition analysis applications autofluorescence avGFP Biochem Biol biological Biophys calculated capillary cence Cerulean channel chapter Chem chromophore coeYcient confocal confocal microscopes coumarin detection detector dipole diVerent donor and acceptor donor fluorescence dyes dynamics electron emission spectra enzyme eVects excitation wavelength filter FLIM fluores fluorescein fluorescence decay fluorescence intensity fluorescence lifetime imaging fluorescence microscopy fluorescence resonance energy fluorophores Fo¨rster frequency domain FRET eYciency FRET imaging FRET pair FRET probes Gadella green fluorescent protein interactions Jovin labeling laser lifetime imaging microscopy linear unmixing lipid living cells measure FRET membrane methods Microsc modulation molecular molecules mutations optical panel pathway peptide photobleaching photon pixel protoplasts pulse quantitative quantum yield quenching ratio resonance energy transfer sample sensitized emission sensors signal spatial spectral images spectroscopy spectrum TCSPC techniques time‐gated tion TIRF Tsien two‐photon variants Venus VFPs wide‐field
Informação bibliográfica
| Título | FRET and FLIM Techniques |
| Editor | Theodorus W. J. Gadella |
| Colaborador | Theodorus W. J. Gadella |
| Edição | revista |
| Editora | Elsevier, 2011 |
| ISBN | 0080915124, 9780080915128 |
| Número de páginas | 560 páginas |
| Exportar citação | BiBTeX EndNote RefMan |