Green Fluorescent Protein: Properties, Applications and ProtocolsSince the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection. |
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Índice
| 1 | |
| 15 | |
| 39 | |
| 67 | |
| 83 | |
6 DISCOVERY AND PROPERTIES OF GFPLIKE PROTEINS FROM NONBIOLUMINESCENT ANTHOZOA | 121 |
7 EVOLUTION OF FUNCTION AND COLOR IN GFPLIKE PROTEINS | 139 |
8 THE USES OF GREEN FLUORESCENT PROTEIN IN PROKARYOTES | 163 |
11 GREEN FLUORESCENT PROTEIN APPLICATIONS IN DROSOPHILA | 227 |
12 THE USES OF GREEN FLUORESCENT PROTEIN IN PLANTS | 259 |
13 USES OF GFP IN TRANSGENIC VERTEBRATES | 285 |
14 THE USES OF GREEN FLUORESCENT PROTEIN IN MAMMALIAN CELLS | 305 |
15 PRACTICAL CONSIDERATIONS FOR USE OF REEF CORAL FLUORESCENT PROTEINS IN MAMMALIAN CELLS APPLICATIONS IN FL... | 339 |
16 PHARMACEUTICAL APPLICATIONS OF GFP AND RCFP | 361 |
17 REASSEMBLED GFP DETECTING PROTEINPROTEIN INTERACTIONS AND PROTEIN EXPRESSION PATTERNS | 391 |
Methods and Protocols | 407 |
9 THE USES OF GREEN FLUORESCENT PROTEIN IN YEASTS | 179 |
10 USES OF GFP IN CAENORHABDITIS ELEGANS | 203 |
Index | 423 |
Outras edições - Ver tudo
Green Fluorescent Protein: Properties, Applications, and Protocols Martin Chalfie,Steven Kain Visualização de excertos - 2006 |
Methods of Biochemical Analysis, Green Fluorescent Protein: Properties ... Martin Chalfie,Steven R. Kain Pré-visualização indisponível - 2005 |
Palavras e frases frequentes
Acad Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chalfie chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics efficient elegans embryos emission energy transfer excitation expressing GFP filter first flow Fluorescent fluorophore FRET function fusion protein gene expression genetic GFP expression GFP fluorescence GFP fusion proteins GFP-like proteins green fluorescent protein Heim identified imaging interactions jellyfish living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy microtubules molecular molecules Morin mutants Natl neurons nuclear peptide photobleaching plasmid Prasher Proc quantum yield RCFPs receptor recombinant red fluorescent protein Renilla luciferase residues screening sequence Shimomura signal specific spectral studies subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrafish
Passagens conhecidas
Página 387 - Miyawaki, A., Llopis. J., Heim, R., McCaffery, JM, Adams, JA, Ikura, M., and Tsien, RY (1997). Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.
Página 116 - Llopis, J., McCaffery, JM, Miyawaki, A., Farquhar, MG, and Tsien, RY (1998) Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.
Página 420 - Cubitt, AB, Heim, R., Adams, SR, Boyd, AE, Gross, LA, and Tsien, RY (1995) Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455.
Página 386 - GL (1996) Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera. Proc Natl Acad Sci USA...
Página 178 - Valdivia, RH and Falkow, S. 1996. "Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction.
Página 120 - Zhang, J., Ma, Y., Taylor, SS, and Tsien, RY (2001) Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering. Proc. Natl. Acad. Sci. USA 98, 14,997-15,002.
Página 282 - Brand, AH and Perrimon, N. (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Página 118 - RY (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Página 137 - Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, and Lukyanov SA 1999. Fluorescent proteins from nonbioluminescent Anthozoa species.
Referências a este livro
Informação bibliográfica
| Título | Green Fluorescent Protein: Properties, Applications and Protocols Volume 47 de Methods of Biochemical Analysis |
| Editores | Martin Chalfie, Steven R. Kain |
| Edição | 2 |
| Editora | John Wiley & Sons, 2005 |
| ISBN | 0471739480, 9780471739487 |
| Número de páginas | 450 páginas |
| Exportar citação | BiBTeX EndNote RefMan |