Nature, Volume 429Sir Norman Lockyer Macmillan Journals Limited, 2004 |
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Página 88
... transfected with COP1 or COP1ARING was extracted , and p53 mRNA levels were assessed by real - time PCR and normalized to B - actin mRNA . d , COP1 increases p53 turnover . HEK293T cells were transfected with or without COP1 , and the ...
... transfected with COP1 or COP1ARING was extracted , and p53 mRNA levels were assessed by real - time PCR and normalized to B - actin mRNA . d , COP1 increases p53 turnover . HEK293T cells were transfected with or without COP1 , and the ...
Página 89
... transfected with Flag - COP1 or Flag - COP1ARING and treated with the proteasome inhibitor ALLN ( Fig . 2e ) . The addition of ALLN markedly increased the level of p53 protein , which was previously reduced by transfection of Flag ...
... transfected with Flag - COP1 or Flag - COP1ARING and treated with the proteasome inhibitor ALLN ( Fig . 2e ) . The addition of ALLN markedly increased the level of p53 protein , which was previously reduced by transfection of Flag ...
Página 92
... transfected with increasing amounts of Flag - COP1 or Flag - COP1ARING with 250 ng pcDNA3.1 + p53 , and U2 - OS cells were transfected with or without increasing amounts ( 0.5 , 1 and 2 μg ) of PCMV - Flag - COP1 or PCMV - Flag ...
... transfected with increasing amounts of Flag - COP1 or Flag - COP1ARING with 250 ng pcDNA3.1 + p53 , and U2 - OS cells were transfected with or without increasing amounts ( 0.5 , 1 and 2 μg ) of PCMV - Flag - COP1 or PCMV - Flag ...
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