NatureSir Norman Lockyer Macmillan Journals Limited, 2007 |
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... BrdU to mice for 1 , 4 or 10 days , and then sorted HSCs onto microscope slides and stained with an anti - BrdU antibody . HSCs were sorted as CD150 * CD48 CD41 lineage Sca - 1 * c - kit cells ( Fig . 2a ) . This popu- lation contains ...
... BrdU to mice for 1 , 4 or 10 days , and then sorted HSCs onto microscope slides and stained with an anti - BrdU antibody . HSCs were sorted as CD150 * CD48 CD41 lineage Sca - 1 * c - kit cells ( Fig . 2a ) . This popu- lation contains ...
Página 240
... BrdU + cells from neonatal or mobilized mice ( % ) DI 58 me N LETTERS The structural basis for activation. chase ( Fig . 3a ) . Thus , these calculations predict that few ( < 20 % ) HSCs should retain BrdU after a 70 - day chase ...
... BrdU + cells from neonatal or mobilized mice ( % ) DI 58 me N LETTERS The structural basis for activation. chase ( Fig . 3a ) . Thus , these calculations predict that few ( < 20 % ) HSCs should retain BrdU after a 70 - day chase ...
Página 241
... BrdU - labeled HSCs Number of cells harvested from individual colony ( > 1 cell ) Colonies with only BrdU - cells Colonies with only BrdU + cells 2 cells 123 86 3 cells 38 50 4 cells 23 26 Total 184 162 Double - positive 27 ± 6 ...
... BrdU - labeled HSCs Number of cells harvested from individual colony ( > 1 cell ) Colonies with only BrdU - cells Colonies with only BrdU + cells 2 cells 123 86 3 cells 38 50 4 cells 23 26 Total 184 162 Double - positive 27 ± 6 ...
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Immunology | 35 |
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